Long-Acting Thyroid Stimulator
نویسنده
چکیده
Highly purified bovine TSH (thyroid-stimulating hormone) was labelled with 125I by using very low concentrations of chloramine-T. Human thyroid membranes prepared by discontinuous sucrose-density-gradient centrifugation were homogeneous on examination by electron microscopy. Incubation of radioiodinated TSH with the membranes showed that radioactivity could be bound to the membranes. Under the experimental conditions described here, binding was dependent on time and temperature and was a saturable phenomenon. Preincubation of the membranes with unlabelled hormone inhibited the subsequent binding of 125I-labelled TSH. Similarly, inhibition by the long-acting thyroid stimulator also showed a saturation behaviour. A rapid and sensitive method for the detection of the long-acting thyroid stimulator is described.
منابع مشابه
Neonatal hyperthyroidism and long-acting thyroid stimulator protector.
Neonatal hyperthyroidism has been thought to result from transplacental passage of long-acting thyroid stimulator (L.A.T.S.) from a mother with Graves's disease. A case is presented here in which no L.A.T.S. was detected in the mother or neonate but another immunoglobulin, L.A.T.S. protector, a specific human thyroid stimulator, was shown to be present in the mother's serum. This stimulator may...
متن کاملHyperthyroidism--pathogenesis and diagnosis.
In the articles below two contributors discuss recent progress in pathogenesis, management, and diagnosis of some thyroid disorders. Dr. Reginald Hall summarizes the evidence that long-acting thyroid stimulator is an antibody, speculating that after reacting with a thyroid chalone it may trigger off Graves's disease. Dr. R. N. Smith discusses management, and emphasizes that recent progress has ...
متن کاملClinical Endocrinology Hyperthyroidism
many as a single entity. The aetiology of the disease remains obscure. It has, however, been clearly established that thyroid-stimulating hormone (TSH) is not responsible for the excessive thyroid activity. An abnormal thyroid stimulator (known as long-acting thyroid stimulator, or LATS), which is both chemically and immunologically distinct from TSH, is found in the serum of many patients with...
متن کاملEffects of long-acting thyroid stimulator (LATS) and LATS protector on human thyroid adenyl cyclase activity.
The long-acting thyroid stimulator (LATS) has been thought to be responsible for the hyperthyroidism of Graves's disease. It is detected by its effect on the mouse thyroid gland but cannot be found in all patients with hyperthyroidism. In an attempt to clarify the problem of LATS-negative hyperthyroidism, serum was obtained from untreated patients and its effect in vitro on human thyroid tissue...
متن کاملInhibition of long-acting thyroid stimulator by thyroid particulate fractions.
The long-acting thyroid stimulator (LATS), found uniquely in the serum of patients with Graves' disease, is closely associated with the immunoglobulin G (IgG) proteins of serum and is removed from serum by antibody to human IgG (2, 3). Since some IgG molecules function as antibodies, it seems possible that LATS is also an antibody. This possibility would be strengthened if LATS could be demonst...
متن کامل